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Integrative Profiling of Metabolites and Proteins 57 57 , Integrative Profiling of Metabolites and Proteins 59 , Retsch mill (ball mill), . Tandem Purification of His6-3x FLAG Tagged Proteins for , Commonly used protein purification methods involve chromatography, , so that it is easy to be broken up and poured into the metal ball mill .

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The proposed standard protocol for protein extraction and solubilization can result in 98% solubilization of brain tissue, corresponding to about 100 μg protein per mg tissue wet weight, by a frozen disintegration/SDS-based solubilization method: Tissue is crushed in the frozen state in a cryotube by shaking with a sterile steel ball.

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Optimized protein extraction from cryopreserved brain tissue , Optimal standard conditions for protein extraction and solubilization from frozen tissue samples have been examined , in the frozen state, by ball mill grinding followed by extraction and solubilization in 2% SDS for 10 min, at 70 degrees C,.

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They recommended a laboratory ball mill with a 9-mm steel grinding ball and disposable 1.2-mL cryotubes to create a deep-frozen powder and then continue RNA, protein and DNA extraction by standard methods.

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We have investigated parameters of brain tissue disintegration, and of extraction/solubilization temperature, time and volume and have reached 98% extraction of brain tissue, corresponding to about 100 µg protein per mg tissue wet weight, by an SDS-based method: Tissue disintegration in the frozen state, by ball mill grinding …

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After ball mill assisted extraction, enrichment of tanshinones and precipitation of protein can be performed simultaneously for sample preparation prior to HPLC analysis, simplifying the process and avoiding blockage of the LC column by protein : [DMIM][Cl] + HPF 6 ↔ [DMIM][PF 6] + HCl.

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